Effect of 405 nm high-intensity narrow-spectrum light on osteoblast function

A significant portion of medical devices fail due to acquired infection, with infection rates after arthroplasty surgery between 1-4%, and considerably higher after revision surgery. To reduce the associated costs of infection, a new preventative method is required. High intensity narrow spectrum (HINS) 405 nm light is a new technology shown to have bactericidal effects on a range of medically important bacteria[1]. The effect of HINS-light on osteoblasts and bacteria were investigated to determine the potential of this technology to contribute to infection prevention in operating theatres, during surgery and postoperative dressing changes

Synergistic efficacy of 405 nm light and chlorinated disinfectants for the enhanced decontamination of Clostridium difficile spores

The ability of Clostridium difficile to form highly resilient spores which can survive in the environment for prolonged periods causes major contamination problems. Antimicrobial 405 nm light is being developed for environmental decontamination within hospitals, however further information relating to its sporicidal efficacy is required. This study aims to establish the efficacy of 405 nm light for inactivation of C. difficile vegetative cells and spores, and to establish whether spore susceptibility can be enhanced by the combined use of 405 nm light with low concentration chlorinated disinfectants. Vegetative cells and spore suspensions were exposed to increasing doses of 405 nm light (at 70–225 mW/cm2) to establish sensitivity. A 99.9% reduction in vegetative cell population was demonstrated with a dose of 252 J/cm2, however spores demonstrated higher resilience, with a 10-fold increase in required dose. Exposures were repeated with spores suspended in the hospital disinfectants sodium hypochlorite, Actichlor and Tristel at non-lethal concentrations (0.1%, 0.001% and 0.0001%, respectively). Enhanced sporicidal activity was achieved when spores were exposed to 405 nm light in the presence of the disinfectants, with a 99.9% reduction achieved following exposure to 33% less light dose than required when exposed to 405 nm light alone. In conclusion, C. difficile vegetative cells and spores can be successfully inactivated using 405 nm light, the sporicidal efficacy can be significantly enhanced when exposed in the presence of low concentration chlorinated disinfectants. Further research may lead to the potential use of 405 nm light decontamination in combination with selected hospital disinfectants to enhance C. difficile cleaning and infection control procedures

Quantifying bacterial transfer from patients to staff during burns dressing and bed changes : implications for infection control

Routine nursing activities such as dressing/bed changes increase bacterial dispersal from burns patients, potentially contaminating healthcare workers (HCW) carrying out these tasks. HCW thus become vectors for transmission of nosocomial infection between patients. The suspected relationship between %total body surface area (%TBSA) of burn and levels of bacterial release has never been fully established. Bacterial contamination of HCW was assessed by contact plate samples (n = 20) from initially sterile gowns worn by the HCW during burns patient dressing/bed changes. Analysis of 24 gowns was undertaken and examined for relationships between %TBSA, time taken for activity, and contamination received by the HCW. Relationships between size of burn and levels of HCW contamination, and time taken for the dressing/bed change and levels of HCW contamination were best described by exponential models. Burn size correlated more strongly (R2 = 0.82, p < 0.001) than time taken (R2 = 0.52, p < 0.001), with levels of contamination received by the HCW. Contamination doubled with every 6–9% TBSA increase in burn size. Burn size was used to create a model to predict bacterial contamination received by a HCW carrying out bed/dressing changes. This may help with the creation of burn-specific guidelines on protective clothing worn by HCW caring for burns patients

Bactericidal effect of corona discharges in atmospheric air

The present paper explores the possibilities of using impulsive and steady-state corona discharges for bio-decontamination operations. A high tension tubular corona electrode was stressed with positive or negative dc voltage with magnitude up to 26 kV, and a grounded mesh was used as an opposite electrode. Different operational regimes of this corona generator were investigated for the production of ozone in air flow and the inactivation of microorganisms. The test microorganisms used in this work were Escherichia coli and Staphylococcus aureus, populations of which were seeded onto agar plates. These bacterial plates were located behind the grounded mesh electrode to assess bactericidal efficacy. The results show that corona discharges have a strong bactericidal effect, for example positive flashing corona discharges were able to reduce populations of the test microorganism by 94% within a 30-60 sec time interval. Negative steady-state corona discharges also produce noticeable bactericidal effect, reducing population of E. coli and S. aureus by more than 97% within 120 sec energisation interval. The bactericidal efficiency of different corona discharge modes and its correlation with ozone levels produced by these discharges is discussed. The results obtained in this work will help in the design and development of compact plasma systems for environmental application

405 nm light exposure of osteoblasts and inactivation of bacterial isolates from arthroplasty patients : potential for new disinfection applications?

Infection rates after arthroplasty surgery are between 1-4 %, rising significantly after revision procedures. To reduce the associated costs of treating these infections, and the patients' post-operative discomfort and trauma, a new preventative method is required. High intensity narrow spectrum (HINS) 405 nm light has bactericidal effects on a wide range of medically important bacteria, and it reduced bacterial bioburden when used as an environmental disinfection method in a Medical Burns Unit. To prove its safety for use for environmental disinfection in orthopaedic theatres during surgery, cultured osteoblasts were exposed to HINS-light of intensities up to 15 mW/cm2 for 1 h (54 J/cm2). Intensities of up to 5 mW/cm2 for 1 h had no effect on cell morphology, activity of alkaline phosphatase, synthesis of collagen or osteocalcin expression, demonstrating that under these conditions this dose is the maximum safe exposure for osteoblasts; after exposure to 15 mW/cm2 all parameters of osteoblast function were significantly decreased. Viability (measured by protein content and Crystal Violet staining) of the osteoblasts was not influenced by exposure to 5 mW/cm2 for at least 2 h. At 5 mW/cm2 HINS-light is an effective bactericide. It killed 98.1 % of Staphylococcus aureus and 83.2 % Staphylococcus epidermis populations seeded on agar surfaces, and is active against both laboratory strains and clinical isolates from infected hip and knee arthroplasties. HINS-light could have potential for development as a method of disinfection to reduce transmission of bacteria during arthroplasty, with wider applications in diverse surgical procedures involving implantation of a medical device. With kind permission of full reproduction from eCM journal (www.ecmjournal.org). Founded by scientists for the benefit of Science rather than profit

Techniques for creating ground-truthed sketch corpora

The problem of recognizing handwritten mathematics notation has been studied for over forty years with little practical success. The poor performance of math recognition systems is due, at least in part, to a lack of realistic data for use in training recognition systems and evaluating their accuracy. In fields for which such data is available, such as face and voice recognition, the data, along with objectively-evaluated recognition contests, has contributed to the rapid advancement of the state of the art. This thesis proposes a method for constructing data corpora not only for hand- written math recognition, but for sketch recognition in general. The method consists of automatically generating template expressions, transcribing these expressions by hand, and automatically labelling them with ground-truth. This approach is motivated by practical considerations and is shown to be more extensible and objective than other potential methods. We introduce a grammar-based approach for the template generation task. In this approach, random derivations in a context-free grammar are controlled so as to generate math expressions for transcription. The generation process may be controlled in terms of expression size and distribution over mathematical semantics. Finally, we present a novel ground-truthing method based on matching terminal symbols in grammar derivations to recognized symbols. The matching is produced by a best-first search through symbol recognition results. Experiments show that this method is highly accurate but rejects many of its inputs

Automated recognition of handwritten mathematics

Most software programs that deal with mathematical objects require input expressions to be linearized using somewhat awkward and unfamiliar string-based syntax. It is natural to desire a method for inputting mathematics using the same two-dimensional syntax employed with pen and paper, and the increasing prevalence of pen- and touch-based interfaces causes this topic to be of practical as well as theoretical interest. Accurately recognizing two-dimensional mathematical notation is a difficult problem that requires not only theoretical advancement over the traditional theories of string-based languages, but also careful consideration of runtime efficiency, data organization, and other practical concerns that arise during system construction. This thesis describes the math recognizer used in the MathBrush pen-math system. At a high level, the two-dimensional syntax of mathematical writing is formalized using a relational grammar. Rather than reporting a single recognition result, all recognizable interpretations of the input are simultaneously represented in a data structure called a parse forest. Individual interpretations may be extracted from the forest and reported one by one as the user requests them. These parsing techniques necessitate robust tree scoring functions, which themselves rely on several lower-level recognition processes for stroke grouping, symbol recognition, and spatial relation classification. The thesis covers the recognition, parsing, and scoring aspects of the MathBrush recognizer, as well as the algorithms and assumptions necessary to combine those systems and formalisms together into a useful and efficient software system. The effectiveness of the resulting system is measured through two accuracy evaluations. One evaluation uses a novel metric based on user effort, while the other replicates the evaluation process of an international accuracy competition. The evaluations show that not only is the performance of the MathBrush recognizer improving over time, but it is also significantly more accurate than other academic recognition systems

Inactivation of C. difficile by 405 nm HINS-light

This poster discusses using 405nm HINS-light to make Clostridium difficile cells and spores inactive

Comparative sensitivity of Trichophyton and Aspergillus conidia to inactivation by violet-blue light exposure

The objective of this paper was to investigate the use of 405 nm light for inhibiting the growth of selected species of dermatophytic and saprophytic fungi. The increasing incidence and resilience of dermatophytic fungal infections is a major issue, and alternative treatment methods are being sought. The sensitivity of the dermatophytic fungi Trichophyton rubrum and Trichophyton mentagrophytes to 405 nm violet-blue light exposure was investigated, and the results compared with those obtained with the saprophytic fungus Aspergillus niger. Microconidia of T. rubrum and T. mentagrophytes and conidia of A. niger were seeded onto Sabauroud dextrose agar plates and irradiated with 405 nm light from an indium-gallium-nitride 99-DIE light-emitting diode (LED) array and the extent of inhibition was measured. Germination of the microconidia of the Trichophyton species was completely inhibited using an irradiance of 35 mW/cm2 for 4 h (dose of 504 J/cm2). Results: A. niger conidia showed greater resistance, and colonial growth developed after light exposure. In liquid suspension tests, 405 nm light dose levels of 360, 720, and 1440 J/cm2 resulted in complete inactivation of T. rubrum microconidia, whereas A. niger showed greater resistance, and at the highest dose level applied (1440 J/cm2 ) although A niger hyphae were completely inactivated, only a 3-log10 reduction of a 5-log10 conidial suspension was achieved. The study results demonstrate the relatively high sensitivity of Trichophyton microconidia to 405 nm violet-blue light, and this is may be of potential interest regarding the control and treatment of dermatophyte infections